Housekeeping proteins for western blot that do not change following nerve injury

Experimental Techniques
1

Hi all,

I need to run a western blot on protein lysate extracted from mouse DRGs. One of my groups has undergone a spared nerve injury and I’m aware that certain commonly used housekeeping proteins can be up or downregulated following injury.

Can anyone recommend me a housekeeping protein that does not change following nerve injury? I am looking 3-weeks post SNI.

I have checked the Renthanl sequencing data set to get some idea of which proteins might be altered follwing nerve injury. Its seems Beta-Tubulin and Beta-Actin would not be a good option because they are upregulated but GAPDH should be ok for normalisation at 3 weeks.

Thanks in advance for your help.

2

Maybe Vinculin?
Much better would be total protein normalization, and then you neednt worry about housekeeping genes changing. https://www.youtube.com/watch?v=N97BQXz4-dY
This is what my lab does. Good luck!

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I do western blots in similar samples and have had luck with GAPDH and total H3 antibodies. Hope this helps.

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