I’m looking at axons from mouse and human DRG in vitro. The standard stain is TUBB3. I’m using the Tuj-1 clone from Biolegend (formerly Covaris), which is the gold-standard as far as I can tell.
I’m using a standard ICC protocol (1% BSA, 0.1% triton in PBS) and 1:500 antibody. In mouse there is faint satellite glia/fibroblast? staining and in human it seems that it is very strong. In the mouse, there are clearly axons, but I think also some non-neuronal cells.
Has anyone else seen this?
Also, what are some good alternatives for specific axon staining in vitro?
adult mouse, DIV7