What fluorescent protein is best for bright, well-defined axonal labeling in culture?
tdTomato is OK, but it’s cytoplasmic and I notice its much brighter in the soma.
My goal is to look at axon degeneration in live cultures (no immunostaining).
ChR2-EYFP from the Ai32 mice looks really nice because it’s membrane-localized. But I don’t want to use ChR2 as the fluor.
What is a good, membrane localized fluor is readily available and looks bright in DRG neurons? Any paper references, especially for axon degeneration stuff, would be great.