I want to stain DRG neurons and skin for CGRP. I’ve tried the goat anti-CGRP from Abcam and sometimes its good, others not so much. Abcam (36001)
I’ve seen the anti-CGRP (rabbit) from Peninsula referenced too, but it’s not produced for me. Does anyone have a really good CGRP antibody?
I’d love to get great staining like this amazing figure form Zylka (2005)
Zylka, M.J., Rice, F.L., and Anderson, D.J. (2005). Topographically Distinct Epidermal Nociceptive Circuits Revealed by Axonal Tracers Targeted to Mrgprd. Neuron 45, 17–25.
In this paper, they use Zamboni fixative and the Peninsula antibody, but I haven’t had such luck. Any tips?