Hi,
I am planning to use DAB with nickel in immunohistochemistery so what is the best concentration that should be used please as I am confused with that?
I was preparing it freshly by adding 2.7 mg in 10 ml PBS (so a concentration of 0.027 % w/v) and then nickel (0.05% w/v) and H2O2 (0.05%) but I did not get a good stain so should I increase DAB to 5-6 mg?
I filter it with 0.45 micro filter before adding H2O2.
I am working on mouse spinal cord sections.
i’ve had good results with this: https://vectorlabs.com/products/dab-hrp-substrate/?gad_source=1&gbraid=0AAAAAD_Ur5gao0LUf2NxUr_phHuzFxbPB&gclid=CjwKCAjwzMi_BhACEiwAX4YZUM6QJY-PNqJ75dbfDh-3Pt_zfInM9KW6nOv012I3ThzWnnHttRbM5xoCLsYQAvD_BwE
See what guidance you might find from their docs.
Hi Alex,
Thanks so much for your reply and advice. I will try this kit.