Whole mount DRG staining

Experimental Techniques
1

Hi everyone, I’m new to pain research and would like to try whole mount stain for murine DRG tissue.

I’m wondering, does the DRG require tissue clearing? If so, any tips for how to physically handle a tissue-cleared DRG? What should I perform the clearing in and how do I transfer this to a slide for imaging?

Thank you!

2

@sshiers @thicunha Any thoughts on this?

3

Hi, sorry I have never performed whole mount staining before on mouse DRG. I’ve been clearing human DRGs, but I don’t have any protocols optimized yet.

4

We did not clear the DRGs, but the wholemount can be visualized in regular confocal microscopy.
You can have this kind of image: Red Nav1.8Tdtomato and Green-Cx3cr1 (both in transgenic background)

image
image1428×1060 474 KB

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5

But, @Niels Eijkelkamp did clearing in this paper: https://www.sciencedirect.com/science/article/pii/S0896627321009697?via%3Dihub

6

Beautiful. Thanks @thicunha

7

Thank you so much everyone. I read here (https://www.sciencedirect.com/science/article/pii/S0165027022000243) a trick to identify tissue-cleared DRG by using a weak UV light to locate a DAPI signal - it worked well.

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