Just another story about how important it is to use the Superfrost Plus:
A colleague came to me yesterday frustrated that during some gentle wash steps, all his spinal cord and brain slices fell off the slide. I asked him if he dried the tissue beforehand, and he said he did, for an hour in fact. At that point, I knew exactly what the problem was. I asked him to show me the slide, and sure enough, it was a regular Superfrost slide, uncharged (not plus). I feel like this happens to everyone at some point. It definitely happened to me before, which is why I’m really vigilant now. So lesson: always check your slides.