Following on the success of this thread
I want to open up a similar one about working with spinal cord.
For making thin mounted sections (14 um) for things like RNAscope, I have previously placed up to 4 spinal cords in cryomold with OCT and then mounted the mold perpendicular to a chuck to make transverse sections.
I directly mount these to a Superfrost slide.
It works OK and I can put several sections per slide. The issues I run into though are inconsistency. Not all slices also mount flat and I also get an issue with air bubbles underneath the tissue section as its thaw mounts to the slide.
I haven’t come up with a great solution to this. I remember @esypek asking about how to avoid bubbles a while back, and I didn’t have a good answer then either.
What is your practice for making thin thaw-mounted cryosections of multiple spinal cords?
I posted a video a few years back about my method. It’s worked pretty well but I want to see if there are improvements I can make to get more sections perfectly cut without bubbles and folding.