How does everyone mount their DRG for cryosectioning?
I was taught by @tberta to put a piece of filter paper down on a chuck, and then lay out a grid array of DRG on the filter paper and then put OCT over it. That’s worked pretty well over the years. Sometimes it’s hard to get all the DRG in one section because of their different geometries, but you can really pack a lot in one section (16-20 for me).
What does everyone else do? Are there other methods people like? Also, is there a case to make for doing one at a time?