Slide storage is becoming a problem in my lab. Typically, we use fixed frozen tissues (DRG/SC) and then do a standard IHC protocol and use fluorescent secondary antibodies. I then use Prolong Gold (Invitrogen) to mount. I usually take images within a few hours to a day. But for long term storage, there are mixed opinions about storing at -20C or at 4C in the dark. What do you do?
@tberta Do you have any thoughts? it’d be easier to store in a cold room in racks than in the -20C space, which is limited.