Dear all,
I am a graduate student currently working on fluorescent immunostaining of mice spinal cord tissue. I am searching for a primary antibody against NR2B that is suitable for immunofluorescence and raised in a host species other than mouse, to avoid background staining from endogenous IgG in mouse tissue.
I have previously tried NR2B antibodies from Abcam for immunofluorescence, but unfortunately, they did not yield satisfactory results. Since my work specifically focuses on fluorescent staining rather than Western blot, I am looking for recommendations that have been tested successfully for immunofluorescence, especially in spinal cord tissue.
If you have experience with a good NR2B antibody that meets these requirements, I would greatly appreciate it if you could share the catalog number, company, and any staining tips.
Thank you very much for your kind help.