Pax2 antibody help

Experimental Techniques Microscopy (IHC/ISH/ICC/In Vivo)
1

Wondering if anyone has had successful staining with Pax2 antibodies?

I’ve tried a rabbit polyclonal antibody from abcam, and a goat polyclonal from R&D systems. I’ve done concentration curves, but I’m finding that even at the lowest concentrations (1:2000), most neurons still seem to be staining for Pax2 (costaining with NeuN). I can reduce concentration even more…just wondering if maybe the antibodies I’m using are no good. Some neurons appear less bright than others, but from what I had read in the literature, it should be clearly positive with Pax2, or nothing at all. Any help on this would be greatly appreciated!

2

@ShanTan

1:500.

This is the good one. Sorry you tried those other ones first.

You can see it here in one of my image panels:

Also, don’t fix too long. Light fixation. 1-2h post fix after your PFA perfusion.

3

I think the rabbit anti-PAX2 antibody from Thermo Fisher Scientific Cat#716000 is better.image is spinal dorsal horn

4

I bought this antibody, this picture is from our lab.

5

Who knows which company has a good TLX3 antibody?

6

@zhzhj131421
The TLX3 ab thay everyone uses in papers is from Carmen Birchmaer’s Lab in Germany. Ask if they will send you some. I asked and they were very generous.

But it’s be nice if there were a good commercial one.

7

Thanks, ALEX. I will try the TLX3 antibody from GeneTex Company.