Looking to see if anyone has had success with fos staining in the spinal cord. I have an antibody that I like, and a protocol that seems to be working okay, but would like to have some improvements. Really trying to do a battery of tests (thermal, chemical, and mechanical assays). Any insight on people’s protocols would be great.
Currently I am anaesthetizing animals and pinching the paw for 30 seconds, and then perfusing 2 hours later. Also want to try this with cold, heat and chemical.