I’m attempting IHC on human DRG/nerve using human serum (containing putative anti-human antibodies).
The issue I’m running into is that there is huge background with just the secondary alone (goat anti-human IgG).
How do people get around this? I know Mouse-on-Mouse background is a thing, but honestly I’ve never seen it despite using lots of mouse antibodies on mouse tissue.
But this human background issue is real. Any thoughts? @sshiers
These are some possible solutions:
https://vectorlabs.com/human-on-human-immunodetection-kit.html
https://www.jacksonimmuno.com/technical/products/groups/fab/blocking