I want to knock down some genes in superficial dorsal horn neurons. I’m avoiding using viral vectors right now and siRNA injection intrathecally looks like an attractive option.
I know that this has been done before, with varying success based on the gene of interest and the transfection method:
What are you experiences and thoughts? I’m going to use siRNA from Dharmacon in mice.
- How many injections (how many days?)
- How many days until I should expect knockdown (3 days, 7 days?)
- What delivery method (PEI, Lipofectamine, naked siRNA?)
- What dose?
I know there aren’t definitive answers to these questions, and I’m going to have to just try stuff and determine empirically, but if anyone has some standard parameters that have worked for them, I’m all ears.