Colocalization analysis of cells - What's the best way? (ImageJ, Photoshop?)

CellProfiler recently came on my radar for cell counting. It looks very powerful.

http://forum.cellprofiler.org/t/rnascope-in-spinal-cord-neurons-quantitation-of-overlap-between-different-in-situ-probes/5355

I recently saw a paper describe using CellProfiler for automated counting of DRGs

https://www.nature.com/articles/s41598-017-01004-y#Sec13

Hopefully we can come up with a reproducible pipeline that works. This would be a huge time saver.