Recently I had to do free-floating immunostaining of spinal cords. In our lab, it’s a laborious and time-consuming procedure. For incubations and washes, most people transfer individual sections from well to well (primary ab -> wash -> wash, etc) by hand using a small hook. This gets good results but it takes forever. The upside is excellent staining and minimal use of antibody-containing solutions.
I know one can use small net-wells. I have this for 24-wells, but it uses a lot more reagent than I’d like.
Does anyone have any other recommendations? Perhaps better fitting netwells or some other kind of chambers?