Recently I had to do free-floating immunostaining of spinal cords. In our lab, it’s a laborious and time-consuming procedure. For incubations and washes, most people transfer individual sections from well to well (primary ab -> wash -> wash, etc) by hand using a small hook. This gets good results bu…

Free-floating immunostaining: tips and tricks?

achamess February 5, 2017, 10:09pm 3

Thanks @lfqueme. I like the netwells but they do damage the slices.

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