Iba-1 and GFAP antibodies

[achamess]

@tberta likes this Iba1 (goat) from Novus

Iba1 vs CD11b for microglia staining?

I agree with Alex - the Iba1 staining is superior and it may due to the quality of the antibodies. However, it is worthy to note that Cd11b/Ox42 is a membrane protein, whereas Iba1 is expressed in cytoplasm. Therefore, Iba1 produce a more plain and robust signal in section with small thickness. A general complain of using Iba1 is that the most popular antibody from Wako (#019-19741) is only made in rabbit, so many researchers have used Cd11b made mouse as an alternative in double staining. Howev…