Iba-1 and GFAP antibodies

[achamess]

If you must use two rabbit antibodies, you can also use tyramide specific amplification

mp20911.pdf

Or you can consider in situ hybridization. I’m a big fan of RNAscope

RNAscope (In situ hybridization) to study gene expression in pain-related tissues

In situ hybridization is a powerful method to interrogate gene expression in tissues. It is known as being a challenging procedure due to the many steps where errors and obstacles arise. We’ve recently begun to use a commercial system called RNAscope from ACD Bio that gets around many of the pitfalls of traditional ISH. I’ve used it a lot now in the spinal cord and DRG. Below is an image of the gene VGAT (Slc32a1) in the dorsal spinal cord. There is nice expression, and most importantly, it was…