We’ve got some experiments coming up, and I’m thinking about testing all the different modalities in a single day. One concern is that performing all these assays will stress the mice out. The other question is the order of assays.
Assays I’d like to perform:
- Von Frey
- Cotton Brush
- Pin Prick
- Cold Plantar
What order would you do these in? And would you separate these across days?
As far as order, generally one moves from less to more noxious. And my rationale for this order is that thermal assays I think can modify/sensitize the tissue more so than the punctate mechanical assays.