Currently, our lab is working on the topical application of capsaicin to the mouse neck area, followed by an examination of allodynia in this region. We are encountering a challenge in ensuring the effective absorption of capsaicin by the skin while preventing inadvertent contact by the mouse forepaw during grooming.
Does anyone have experience with topical capsaicin application to mouse skin and could kindly provide some guidance on the process? For instance, are the mice subjected to anesthesia, or is any form of restraint employed? Additionally, I am curious to know whether the capsaicin solution needs to be rubbed for a specific duration until the skin fully absorbs it, and if so, for how long?
The paper I found on topical capsaicin on animals lacks details on how the capsaicin was applied topically. I have come across papers with human subjects where gelform or patches were used. However, mice cannot control themselves from grooming and touching the application region.
Any insights or advice you can share on these matters would be greatly appreciated.