Does anyone have any experience using adult DRGs, either as explants or dissociated cultures, for axonal degeneration studies? My preference is to use adult because the process/disease I want to study occurs in adults, and also I’d like the full mature repertoire of non-neuronal cells like SGCs, macrophages, etc. to be present. I don’t know if the neuropathic process I want to study is a ‘neuron intrinsic’ one or if it requires other cells, so I don’t want to just have neurons all alone in a dish.
Most all papers use embryonic DRGs. I’m assuming because they grow more readily and so you have more axon to quantify. But apart from that, any other reason that adult DRG shouldn’t work?
I did find a few recent papers using adult DRG:
- They use adult DRG in Xona microfluid chambers and look at axon fragmentation
But, in contrast, this paper from Neto et al. suggests adult explants don’t grow much axon
Latremoliere et al. adapted the “spot culture” method that is normally used with embryonic DRG and got it going with adult DRG