Digital vs real-time for qPCR

Experimental Techniques Reagents and Equipment
1

Does anyone have experience using digital PCR who can describe pros and cons between digital and real-time machines?

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I haven’t tried. What’s got you thinking about this? Did you decide to do this?

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No, we decided to go with a real-time machine that can multiplex Taqman assays. dPCR is very expensive, it does provide absolute quantification but usually we just need fold change data.

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In case anyone is interested, there is a Qiagen webinar this Wed comparing real-time PCR to digital PCR and advantages for multi-plexing (I have no connection to Qiagen). It will be available on demand:
https://www.qiagen.com/us/knowledge-and-support/knowledge-hub/events-and-webinars/webinars/q-rious?cmpid=CM_QriousShow_additional_March_dPCR_PCR_WW_0323_7960_13066&elq_cid=2376432&elq_mid=13066&utm_source=CM_QriousShow_additional_March_dPCR_PCR_WW_0323&emhash=495abfe331eeacf9ee8e90817c2a8e8c

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