Fos staining in the spinal cord

[achamess]

FosGFP Mice for Noxious Stimulation - Some results

For anyone who is interested, I recently piloted using noxious heat stimulation (55C for 30 s) in a FosGFP animal (Barth 2004). https://www.jax.org/strain/014135 Looks pretty good. Fos IHC is superior no doubt, but that has been reported. For electrophysiology, this is useful. I perfused 2 hours after stimulation. [image]

Standard IHC staining. Nothing special. This is the Santa Cruz Fos antibody which isn’t made anymore. 90 mins to 2 hrs is typical time.

Do you have an antibody?