We have tested several GFAP Abs and none really worked well, and we always go back to the old but still the best MAB360 ( Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 ascites fluid, clone GA5, Chemicon® | Sigma-Aldrich (sigmaaldrich.com)).
Using this Ab, we have showed that naïve SGCs do not express GFAP (or only a few) and this is in agreement with our and other single-cell data.
See an example of the IHC using the MAB360 ( 1/500, BSA1%, vehicle ← vs nitroglycerin → ).
