Reference for Size Classification of Dorsal Root Ganglia Neurons in Mouse

achamess · May 5, 2018, 1:02am

Does anyone have a paper that classifies mouse DRG neurons based on size? That is, what is considered “small size”, "medium size’, “large size”?

@tberta @liz

achamess · May 6, 2018, 12:24am

I found one recent paper that provided a size classification. It seems reasonable:

< 300 um = small
300-600 um =medium
600+ um = large

zhzhj131421 · May 6, 2018, 3:47am

Alex is right, but someone thinks 400-600 is medium in mice.

tberta · May 8, 2018, 1:41pm

This is the paper that I always use as reference for size classification of murine DRG neurons:

ncbi.nlm.nih.gov

Modification of classical neurochemical markers in identified primary afferent neurons with Abeta-, Adelta-, and C-fibers after chronic constriction injury in mice.

R Ruscheweyh, L Forsthuber, D Schoffnegger and J Sandkühler, The Journal of comparative neurology, May 2007 10

It is functionally important to differentiate between primary afferent neurons with A-fibers, which are nociceptive or nonnociceptive, and C-fibers, which are mainly nociceptive. Neurochemical markers such as neurofilament 200 (NF200), substance P (SP), and isolectin B4 (IB4) have been useful to distinguish between A- and C-fiber neurons. However, the expression patterns of these markers change after peripheral nerve injury, so that it is not clear whether they still distinguish between fiber types in models of neuropathic pain. We identified neurons with Abeta-, Adelta-, and C-fibers by their conduction velocity (corrected for utilization time) in dorsal root ganglia taken from mice after a chronic constriction injury (CCI) of the sciatic nerve and control mice, and later stained them for IB4, SP, calcitonin gene-related peptide (CGRP), NF200, and neuropeptide Y (NPY). NF200 remained a good marker for A-fiber neurons, and IB4 and SP remained good markers for C-fiber neurons after CCI. NPY was absent in controls but was expressed in A-fiber neurons after CCI. After CCI, a group of C-fiber neurons emerged that expressed none of the tested markers. The size distribution of the markers was investigated in larger samples of unidentified dorsal root ganglion neurons and, together with the results from the identified neurons, provided only limited evidence for the expression of SP in Abeta-fiber neurons after CCI. The extent of up-regulation of NPY showed a strong inverse correlation with the degree of heat hyperalgesia.

. < 300 um = small
. 300-700 um = medium
. > 700 um = large

achamess · May 8, 2018, 1:58pm

Thanks Temo. I really like that paper.

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Reference for Size Classification of Dorsal Root Ganglia Neurons in Mouse

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