Costaining with IHC or another RNAscope probe? I’d use Cy3 for Trpv1 and Cy5 for your GOI because we’ve found that has the lowest autofluorescence. Cy3 is brighter but it may be hard to find real signal if your gene is a low expresser. You can get a sense of the expression of your gene from gene expression databases or comparing your qPCR for GOI to another gene whose expression you have an impression about. We like the V2 kit. I’d use that.