RNAscope (In situ hybridization) to study gene expression in pain-related tissues


#41

Hi @achamess, thanks for all your helpful input on this forum. I’m trying to do RNAscope in a tdTomato mouse line - could you advise on how to recover the fluorescence with immunolabelling after the RNAscope procedure?
Thanks!


#42

@sarah.chng

https://rockland-inc.com/store/Antibodies-to-GFP-and-Antibodies-to-RFP-600-401-379-O4L_24299.aspx

1:500 after RNAscope. @MGM has had success with this.

Good luck.