RNAscope (In situ hybridization) to study gene expression in pain-related tissues


Hi @achamess, thanks for all your helpful input on this forum. I’m trying to do RNAscope in a tdTomato mouse line - could you advise on how to recover the fluorescence with immunolabelling after the RNAscope procedure?




1:500 after RNAscope. @MGM has had success with this.

Good luck.