Hello. I am trying to use intersectional strategies to target VPL Projecting neurons from the spinal cord.
My original plan was to intraspinally inject cre dependent DREADD around p15-20 and then inject AAV retro Cre in the brain around P42 to selectively express the DREADD.
However, I am concerned if the order of injection matters in this case? Would the spinal cord neurons be infected with the DREADD virus still without the CRE and get later expressed?
I have read papers that inject the two viruses at the same time in different brain regions but don’t know if having a long term between injections would change the results.
Any tips or advice would be appreciated!