Does any one have any thoughts about the performance of different AAV serotypes (and promoters) in the spinal dorsal horn?
My aim is to transduce dorsal horn neurons with intraspinal injections using a pan-neuronal promoter (Synapsin). I used AAV9 with a synapsin-driven gene and I saw really high transduction of deep dorsal horn neurons, but not lamina II, which was quite strange. I’m not sure if it’s this particular gene (a fluorophore) or the serotype (AAV9) or the injection method (maybe we went too deep?).