Intrathecal Bicuculline/Strychnine as a model of mechanical allodynia in mouse?

I want to induce mechanical allodynia in a reversible way. Capsaicin is one way, but I’ve also looked at bicuculline/strychnine, which are GABA and glycine antagonists, respectively.

In this paper by Cheng et al. they used 20 ng Bicuc and 50 ng strychnine intrathecally.
I tried this dose today, but mechanical hypersensitivity wasn’t apparent at 1 hour. I think maybe I waited too long to test. The time course might be very short.

As suggested by this paper, peak effects were in 10 mins

04 PM

That doesn’t give you much wiggle room to do your testing and inject a whole cohort of mice.

I saw in Francois 2017, they used 1 ng bicuculline, which is much less than Cheng 2017. The time frame is not indicated (AFAIK)

37 PM

So does anyone have experience using these agents in mice?

@liz @lfqueme @tberta @SamineniV @Boninrp