Mortar and pestle for grinding mouse DRG in liquid nitrogen?


#1

Does anyone have recommendations for a small mortar pestle that is sufficient for liquid nitrogen grinding of mouse DRG? There are plenty of mortar and pestles but they’re large, probably too large for a few DRG. Anyone do this?


#2

We use a homogenizer with plastic pestles–it works great for small tissue stored in eppendorf tubes.

https://www.fishersci.com/shop/products/kontes-pellet-pestle-cordless-motor-1/k7495400000#?keyword=Pellet+pestle+mixer


#3

Do you use with liquid nitrogen? We have this. I didn’t think it’d work for my purpose.


#4

Not sure what exactly you mean. We don’t have liquid nitrogen in the tubes, but we flash freeze in liquid nitrogen, store at -80, and then use the homogenizer with the Qiagen RNAeasy kit for RNA isolation. I think it should be fine for standard trizol protocols as well


#5

Hey. OK. Yeah, no I’m not trying to extract RNA. For certain applications, people will flash freeze tissue and then grind the tissue while it’s still frozen solid (in liquid nitrogen) to get a fine powder.

Like this:

DRGs are so tiny, I’m worried if i used a standard sized mortar pestle, it’s spread all over and I’d recover very little.

@tberta - have you done this?


#6

Ah, okay. I don’t have experience with that, but you may be able to use a glass tube mortar and pestle, like this one: https://www.supplymylab.com/Supplies/Mortar-and-Pestles/_/64AD585F-1B65-4162-9023-BBA71281498E?q=099C%20S65&gclid=EAIaIQobChMI9Yz8zdSP2gIVVsDICh3vzgYiEAQYBSABEgLiEvD_BwE


#7

We use this technique for skin samples, but a simple Trizol and pestle approach works fine for DRG and spinal cord.


#8

Thanks Temo. Yes, for just getting RNA out, mortar and pestle (glass), as you showed me, works well. But I want to do frozen with liquid nitrogen to do other things (nuclei extraction). We’ll see.