Wondering if anyone has had successful staining with Pax2 antibodies?
I’ve tried a rabbit polyclonal antibody from abcam, and a goat polyclonal from R&D systems. I’ve done concentration curves, but I’m finding that even at the lowest concentrations (1:2000), most neurons still seem to be staining for Pax2 (costaining with NeuN). I can reduce concentration even more…just wondering if maybe the antibodies I’m using are no good. Some neurons appear less bright than others, but from what I had read in the literature, it should be clearly positive with Pax2, or nothing at all. Any help on this would be greatly appreciated!