Resiniferatoxin administration protocol

I’m looking for a resiniferatoxin administration protocol for adult animals to chemically desensitize peptidergic neurons. Any pluses, minuses, or caveats (for administration or publication using this procedure) are greatly appreciated as I have only read about it and never performed this procedure before.

Thanks all!

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Hi @Taves
Thanks for the question. I’ve asked some colleagues to jump in the discussion. But I had this paper in my library that goes into detail for RTX ablation, but in rats.

This one is in the mouse with direct injections or intrathecal.

Good luck!

Hi Taves,
Resiniferatoxin (RTX, 1 mg/kg body weight) was given daily by I.P. totally for 3 days, and mice were sacrificed 24 h after the last injection. It work well on mice. Good luck!

@Taves Here is another protocol that seems to be exactly what you’re looking for. It’s from @YongHo too.

RTX Ablation of Peripheral TRPV1-Expressing Neurons: Three- to four-week-old mice were intraperitoneally injected with RTX dissolved in a mixture of 10% Tween-80 and 10% ethanol in normal saline or vehicle alone under isoflurane anesthesia as a single bolus in two injections of 50 μg/kg and 150 μg/kg on days 1 and 2, consecutively. RTX-treated mice were used in experiments at least 7 days after final RTX injection.

TRPV1 in GABAergic Interneurons Mediates Neuropathic Mechanical Allodynia and Disinhibition of the Nociceptive Circuitry in the Spinal Cord