Simplified Production of Adeno-Associated Virus from HEK293T Cells


Here is a simplified protocol that I use to make adeno-associated virus (AAV) in house. It’s based on protocols from Lock et al. 2010 and Vandenberghe et al 2010. We generally make AAV9 serotype, which is released very efficiently into the medium, so that is why we collect the medium here.

If you have any questions, let me know.

AAV Protocol