# Pain Single Cell
Collections of Papers and Notes on Single Cell Studies in Pain Research
## Spinal Cord scRNA-seq Studies
- Delile, J., Rayon, T., Melchionda, M., Edwards, A., Briscoe, J., and Sagner, A. (2019). Single cell transcriptomics reveals spatial and temporal dynamics of gene expression in the developing mouse spinal cord. Development 146, dev173807. [#Delile:2019go]
- Häring, M., Zeisel, A., Hochgerner, H., Rinwa, P., Jakobsson, J.E.T., Lönnerberg, P., Manno, G., Sharma, N., Borgius, L., Kiehn, O., et al. (2018). Neuronal atlas of the dorsal horn defines its architecture and links sensory input to transcriptional cell types. Nat Neurosci 61, 1.[#Haring:2018dx]
- **Method**: Fluidigm C1 Single **Cell** (not nuclei) RNA-seq from adult mouse spinal cord. Enriched dorsal horn neurons using Vglut2-EYFP and Vgat-EYFP
- **Findings**:
- Defined **30 neuronal types**
- 15 GABA/15 GLUT (w/ one dual)
- Verified mRNA expression using multiplexed RNAscope
- Identified neuronal types responsive to heat and cold using _Arc_ expression by RNAscope
- Rosenberg, A.B., Roco, C.M., Muscat, R.A., Kuchina, A., Sample, P., Yao, Z., Gray, L., Peeler, D.J., Mukherjee, S., Chen, W., et al. (2018). Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding. Science 12, eaam8999.[#Rosenberg:2018it]
- **Method**: SPLIT-seq (method development paper)
- **Findings**:
- Identiified multiple known and unknown spinal cell types
- Massive number of cells.
- Mixed the entire spinal cord, not just a single level.
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