Transgenic Cre or other reporter lines for specific labeling of sensory, motor and autonomic neurons in the periphery

I’m looking into mouse lines for labeling of sensory, motor and autonomic neurons.

For sensory, Advillin-Cre has been a go-to, but it’s specificity has been called into question

For autonomic, I’ve seen TH-Cre used, but TH is also in C-LTMRs.

For motor, one can use ChAT-Cre, although some autonomic should also have ChAT.

Is there any method or mouse line that can specifically label a single population?

@tberta @esypek @liz @thicunha

Collecting some responses from Twitter. Check it out.

Pirt-cre is a good alternative to Advillin, see images of DRG and skin tissues obtained recently in our lab.


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Oooh that’s pretty! Did you check spinal cord and other areas? Is Pirt also in sympathetic and motor?

A PhD student is characterizing these mice right now, and I will keep you posted. However, we obtained these mice from Qin Liu and she may have more information - just knock at her door!

We used Nav1.8-Cre mice to specifically target C-fibers, for deleting NMDA receptors:

and for deleting mu-opioid receptors:

In the first paper, peripherin-Cre mice were also used. Peripherin labels all primary afferent neurons.

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