FosGFP Mice for Noxious Stimulation - Some results


#1

For anyone who is interested, I recently piloted using noxious heat stimulation (55C for 30 s) in a FosGFP animal (Barth 2004). https://www.jax.org/strain/014135

Looks pretty good. Fos IHC is superior no doubt, but that has been reported. For electrophysiology, this is useful. I perfused 2 hours after stimulation.


Stuff I tried during grad school/post-doc that won't get published but could be useful
#2

Is that the PC-38 antibody for cFos? GFP looks nice. What did without heat stimulation look like? Thanks for sharing this.


#3

@RPSeal Contra side and also no stimulus control had very little signal. Maybe a single random GFP+ cell. Also, I should add that this is the native GFP fluorescence. I’m sure it would look much better with anti-GFP antibody, but that would be unrealistic for using it live. This is the Santa Cruz goat anti-Fos sc-52 (the original one, before they stopped selling it). This is also 14 um mounted slice, not free floating. Overall I’m satisfied with this.


#4

Ok. Sounds good! Thanks again Alex. Very nice.