Stuff I tried during grad school/post-doc that won't get published but could be useful

[achamess]

I talked about this in another thread

FosGFP Mice for Noxious Stimulation - Some results

For anyone who is interested, I recently piloted using noxious heat stimulation (55C for 30 s) in a FosGFP animal (Barth 2004). https://www.jax.org/strain/014135 Looks pretty good. Fos IHC is superior no doubt, but that has been reported. For electrophysiology, this is useful. I perfused 2 hours after stimulation. [image]

Here are some more images. Overall, this FosGFP line is good for activity-dependent labeling. It’s not perfect. There are some Fos+ (IHC) neurons that don’t get labeled, but overall, it’s useful. Could be good for electrophysiology.

The stimulus here was 55ºC heat for 30s, followed by perfusion 2 hours after the stimulus.