Late to the party, but I just wanted to add that Beta3 tubulin and Actin always worked well in my hands. In my experience, most of the variation more often comes from the dissection, meaning how much nerve is left attached to the DRG. We identify the part of the DRG containing the neuronal bodies (it is kind of transparent) and we quickly trim around that. Keeping this consistent is also key for getting good, healthy cultures.
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