FYI: Here is a recipe from @zhzhj131421 : http://www.bio-protocol.org/e2020
Prepare the glass capillary: the glass capillaries are pulled by a P-97 micropipette puller with the heat: 850, vel: 30, and time: 250.
Trim the tip of the glass capillary tube with scissors under operating microscope, so that the tapered tip has an outer diameter of about 50 μm.
Insert the tip of glass microliter syringe (type of volume 10μl) into the glass capillary, and seal the junction with hot glue (Video 1).
Fill the glass microliter syringe and glass capillary with liquid paraffin, evacuate the air in them.
Fix the microinjection set on the desktop digital stereotaxic instruments.