I am looking to perform an assay for cold sensation to evaluate a model of immune-mediated neuropathy.
I suspect that the treatment will make the animals more sensitive to cold stimuli, but things could go either way so I want a test that can see both increases and reductions in cold sensation.
I also want an assay that is not too onerous to perform and has as clear a behavior as possible (minimize subjectivity).
Options I’ve seen include:
- Acetone test
- Cold plate assay
- Latency to nocifensive behavior
- Number of flinches over 5 min period @ 10ºC (see below)
- Cold plantar test
- Dynamic Cold Plate Test
What assays do you prefer? Which do you have most confidence in?
At the moment, the cold plate test with counting nocifensive behaviors (recorded with video) seems most appealing to me. But I’m open to anything.