Spinal Cord Staining thickness

Hi everyone,

I’ve been staining 30um slices for spinal cords (lumbar) - From your experience, is it better to do thinner slices?

Slide mounted or free floating?

Mounted : 14 um
Free floating: 40 um

Free floating. Thanks, I’ve been cutting at 30 um. Thought maybe I could go a bit thinner.

You can get away with 30, but for free floating, I wouldn’t go thinner. In the literature, you normally see 40-60 um. As you get thinner, ripping or tearing will be come an issue. if you want very thin sections, you need to do mounted slides.