EF1a promoter to drive expression in DRG in vivo?

[achamess]

Blast from the past - here is Syn in DRG that I did intrathecal. It’s clearly there, just not as much as you might need for your application. Never did EF1a

Stuff I tried during grad school/post-doc that won't get published but could be useful

Viral vectors What I tried: I injected AAV9-hSyn-EGFP (from Penn) into the plantar hindpaw of adult mice. The virus was 10^13 vg/ml. I injected 10 ul. I saw some (~10%) transduction in the L4 DRG after injection 3 weeks later. It wasn’t impressive. Thoughts: AAV9 is known to retrogradely transport, but the efficiency is probably low. There is likely more infection that I can detect with fluorescence microscopy, but the expression is too low to be noticeable. Other serotypes such as AAV6 may be…

@SamineniV Have you checked EF1a in DRG?